PI0066 -------LNLTLNENRIYGLLGKNGMGKSTLLYLISGL--FKHLSMGQKKKIYMSFAVASCCQLLLMDEPTNGLDIPSK-

PI0106 PKWVLRNINLIIPKGKTIALVGQSGSGKSTLLDLIPRY--GGRLSGGQRQRVSIARAILKNPPILILDEATSALDTESE-

PI0110 FSPVVTDINVEIKSGELTCLIGSNGIGKSTLLKTLTGF--VQTLSDGERQKVMIAKALAQQTPIILLDEPTSFLDFPSKV

PI0226 KGFKMKDLTFNVPERTVTALVGSSGSGKTTITNLLLRF--GLGLSGGQKQRLSIARAFLKDAPILLLDEITSNVDPVNE-

PI0227 KDNALKDVCLQFPRGSHTAIVGESGSGKTTLASLMMGF--GVKFSGGEKQRISIARMLLKDSPIVILDEATAALDGENE-

PI0531a KVQALRDVSFSVGKGEVFGLIGPDGAGKTSMYRILCSL--AGALSGGMKQKLALCCALVHQPSVLFLDEPTTGVDPVSR-

PI0531b TFRAVDDISFTVKKGEIFGFLGANGAGKTTAMHMLTGL--VSNLPLGWKQKLAFSVSIFHEPGVVFLDEPTGGVDPATR-

PI0734 EKQVLHNVSFTARQGTVTALVGPSGGGKSTTAKLAARF--GETLSGGERQRISIARALLKNAPIILLDEATASLDAENE-

PI0735 ERDAVNHIDLDIPQGKTVALVGASGSGKTTIARLIPRF--GTYLSGGEQQRIVLARAILKDAPIVVLDEATAFADPENE-

PI1134 GTVLFDHVNFNVEKEQKVVFLSRNPKAMTALFNIINGE--VNVLSGGEKMRCMIARMQLQNANCLILDTPTNHLDLESI-

PI1140a VKPLFHNVSFVVNDRDRIALVGKNGAGKSTMLKILCGL--TSEFSGGWRMRIELAKILLRRPDVLLLDEPTNHLDIESI-

PI1140b PRLVFDKVDMTIKRGEKVAFVGRNGEGKSTLVKCIMG---VKVLSGGERSRLAMIKLLLEPVNLLILDEPTNHLDITSK-

PI1607 ---VLKGIDLRIEKGEIVSIVGPSGAGKTTLLQILGTL--PNELSGGEKQRVAVARALMNNPAVILADEPSGSLDSKNKE

PI1709 NKEVLHDINTKFEDGKTNLIIGQSGAGKTVLVQCLVGL--PGEISGGMQKRVAIARAVVMNPKYLFCDEPNSGLDPKTSL

PI1711 KRTVANGVSINVRQGEIVGLLGPNGAGKTTSFYMTTGL--GDRLSGGERRRCEIARCLAIDPKFIMLDEPFAGVDPIAV-

PI1713a KQILKN-IYLSFFYGAKIGIIGLNGAGKSTLMKIIAGI--VTHLSGGERRRVALCRLLLQKPDVLLLDEPTNHLDAESI-

PI1713b EKVLFSDLNFMLPPNGIVGVIGPNGAGKTTLFRLIMGL--CGVLSGGERNRLQLALALKQEGNVLLLDEPTNDIDVNTL-

PI1822 EDNVIEHLNYDFYPGSCTAILGETGSGKTTMVRMILAL--GGGLSEGQAQRISIARALLRNRSIMLFDEATSALDPDTER

PI1951 DKEILKGIDLTVKDGEIHAIMGPNGSGKSTLSAVLTGNPLNEGFSGGEKKRNEIFQMAMLQPKLSILDETDSGLDVDAMR

PI2008 KVNILSGINGVGKSTILNKVIKGLSQGGEFPSHMMKGVHIIDELFAETGKKIVRSENEIRFSQVGETLFPYQLSSGEKQ-

pPI0278 QNTVIDIPELTLHKGELIGLVGNNGAGKTTLMRLILDL--IRDFSEGNRQKIGIIGAMIVNPEILILDEPFNYLDPSSQI

pPI0504a AQILFDEISFSIAEGQHVGLIAKNGTGKSTLLSILTGK--MGQLSGGQQKRVALANVLILEPDLLILDEPTNHLDLEMI-

pPI0504b EKVILKDFYYNFARFEKMGIVGNNGTGKSTFIKMLLGL--VAKLSGGEKRKLYLCTVLMRNPNFLILDEPTNDLDIQTL-

pPI0565 EKQVLHNVSFTARQGTVTALVGPSGGGKSTTAKLAARF--GETLSGGERQRISIARALLKNAPIILLDEATASLDAENE-

Walker A Signature Walker B

PI0066 ---ALFRKVIAGNLPADSSLIIS---THQVHDVEQLLDHVFVLNNSEMIINASV

PI0106 ---RLVQDALYKLM-KTRTTIAV---AHRLSTIKNSDE-ICVMHEGEIVERGTH

PI0110 EMLQLLHRLAKETDKVVFLSTHD---LELALRIADLLVELDKSGLHVVPAEKVQ

PI0226 ---YKIQQAMSALI-RNRTVLVI---AHHLQTIRNAHQ-IIVMDKGRLMEKGTH

PI0227 ---KLIQEALDELQ-RNKTVITI---AHRLNTIQDMER-IVVMDKGQVVSKGTH

PI0531a ---KEFWEILAQLKARDITIVAS---TPYLDEVRSCER-VAFLSEGIVRGIGTP

PI0531b ---RQFWELIYDAAERGITVFVT---THYMDEAEYCDR-ISIMVDGKIKALGTP

PI0734 ---TKIQAGISELV-RNKTVIII---AHRMRTVRNADH-IVVLSGGTVSEQGTP

PI0735 ---HLIRQAFAHLT-CGKTVLMI---AHRLTTVQDADN-IVVVDNGRIAEQGTH

PI1134 ---QAFNNNLVGFKGNILFSSHD---HEFIGTVANRII-ELT-PNGTIDKLMSY

PI1140a ---QWLEQFLAQSAKAVVLVSHD---RAFINNVTNRTL-EIT-CGRVEDYKVKY

PI1140b ---EVLKEAIKAFDGTAIIVSHD---REFLDGLVSKVY-EFGGGKVREHLGGIY

PI1607 ELHKLFFELRDKFGQTFVIVTHD---ETLAT-LTDRTI-HLK--DGRIVGEGNE

PI1709 VIDELLSDITREFNITTIINTHD---MNSVMGIGENIV-FIA--DGRAEWQGNK

PI1711 ---EDIQHIVWRLKFRNIGILIT---DHNVHETLNITDRAYLLFEGQILFKGTP

PI1713a ---DWLEQHLQQYEGTVIAVTHD---RYFLDDVSEWIL-ELD-RGEGIPWKGNY

PI1713b ---RALEEGLENFAGCAVVISHD---RWFLDRICTHIL-AFEGNGEVFFFEGTY

PI1822 ---ELLQNILSKHNKTIIFITHR---PAVVEYCDQTIEVRKQHSHREYIKRGAG

PI1951 -IVADGVNKMHTSETSTIVITHY---DRLLDMIKPSVV--HILYNGRIVKTGGP

PI2008 -ILAILLTVLVQDGLPYVLFMDEPEVSMHVDWQKKLIALILSLNPNVQIILSTH

pPI0278 NIARIIHEVNQQHGTTVLISSHN---LNFISEICNRIILMEKGVILKDLTNADE

pPI0504a ---EWLEGFLSRGNKTLLMVTHD---RYFLDNVCNIIL-ELD-DRTIYTYRGNY

pPI0504b ---QILEEYLQDFPGCVIVVSHD---RYFMDKVVDHLL-VFNGDGQIKDFPGNY

pPI0565 ---TKIQAGISELV-RNKTVIII---AHRMRTVRNADH-IVVLSGGTVSEQGTP

Downstream Region