10 20 30 40 50 60 70 80 90 100

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

NG0170 1 VFGPNGAGKSTFLKSLMGLQPIDTGSIRLDG--LTRQNIAYLP---------QQSDI--DRSQPMTVF----DLAAMGLWYEI--GFFKGINTAQKQR-- 79

NG2088 1 LIGPNGAGKSTLFSFMARLRPLESGSIAYRGKNLAGTPTAELA---------RTLSILTQENSIMSRI----TVRDLLMFGRY--PYHQGRPTAECRR-- 83

NG0192 1 LLGSSGSGKSTLLRMLAGMESPNQGKIILDGQDI----TKLAPY-------DRPINMMFQSYALFPHM----TVEQNIAFGL----KQDKMPKGEIDA-- 79

NG0445 1 LLGPSGGGKTTLLRIIAGLENADGGQILFDGQDV----TAKHVR-------ERKVGFVFQHYALFRHM----NVFDNVAFGLTVLPKPERPSKGQIRA-- 83

NG2039 1 VLGRSGCGKSTLLNMIAGIVRPDGGEIRLNGENI----TCMPPE-------KRRISLMFQDYALFPHM----SALENTAFGL----KMQKMPKAEAER-- 79

NG0215 1 IIGASGCGKTTLLRCLAGFEQPDSGEISLSGKTIFSKNTNLPVR-------ERRLGYLVQEGVLFPHL----TVYRNIAYGL----GNGKGRTAQERQ-- 83

NG0374 1 ILGPSGSGKTTFLRCLNALEMPEDGQIEFDNARPLRIDFSKKTSKHDILALRRKSGMVFQQYNLFPHK----TVLENVMEGPV---AVQGKPAAQARE-- 91

NG2013 1 LLGPSGCGKSTLLRCVNGLEPHQGGSIVMDGVGEFGKDVSWQTA-------RQKVGMVFQSYELFAHM----TVIENILLGPV---KVQNRDRAEAEA-- 84

NG2137 1 LMGYSGAGKSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQA------RQNIGMVFQQFNLLSNR----TVAGNVAF-PL---EIAGWPSEKIKA-- 84

NG2116 1 VMGGSGSGKTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEH------RRRMGVLFQHGALFTDL----SVFDNIAFPMR---ELTQLPEAVIRD-- 85

NG0770 1 IIGSSGSGKSTLLHILGGLDMPSEGRVLLMGEDLRALNQRRLGDLR-----NRHLGFVYQFHHLLPEF----SALENVMMPL----LIGKKSRKEAVE-- 85

NG1922 1 IAGHSGSGKSTVLKLISGITKPSMGKVLFNGQDLGTLSDNQIGFMR------QHIGIVFQDHKILNDR----NVLQNVILPL----RIIGYPPRKAEE-- 84

NG1439 1 IIGQSGSGKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALR-----RERFGFIFQRYNLLSSL----TARDNVALPA----VYMGMGGKERSA-- 85

NG1605 1 LLGPNGAGKTTGFYMIVGLIAADAGSVMLDGRELRHLPIHERA--------RLGVGYLPQEASIFRKM----TVEQNIRAIL----EIRTKDKNQIDR-- 82

NG1399 1 LAGHNGAGKSTIMKLILGLITPTEGEVMLLGERTGSKAGARL---------RSQIGYLPETVALHPSL----TGIETLDF--------YAKLKKQPLT-- 77

NG1732 1 LIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESL---------RAQIGLVTQDTSLL-HR----SVRDNIIYGRPDATDAEMVSAAERAE-- 84

NG2165 1 LVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCL---------RAQFALVSQDVFLF-DD----TLFENVRYSRPDAGEAEVLSALQAAN-- 84

NG1434 1 LFGPSGCGKTTVLRLIAGLETPKSGTIRNTF---------------------HKTGFLFQENRLPENL----TAMQNIAI------FMDNPDEGEIVA-- 67

NG0764 1 LIGANGSGKSTFMKILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAIYANPEATEDDY----MKAAELEAKFAEYD 96

NG0781 1 LIGKNGTGKSSLFALIKGEITQDGGDISIPKNWRLASVSQETPDL-DISALDYVLQGDAELQAFQTA-----LRQAEVQNDG----MKQAEYHAKLEEID 90

NG0870 1 LIGRNGAGKSSFLKILTGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYHHVSHELENGSSEL--LLKELNELQLEIEA-- 96

NG1573 1 LLGLNGTGKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQKRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGS 100

NG0764 1 IIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK------------ASVGYYPQDH--ENDFD---VDMDLSE---------WMRQWGREGDDE 74

NG0781 1 LLGVNGSGKSTFIKALAGKID------LL--SGSIVHSEK------------LNIGYFAQHQ--LDTIR---ADQSPVW---------HIQQLSPEVR-E 65

NG0870 1 LIGPNGIGKTTFLKLILGELQP--------TYGRIRIGSK------------QEVAYFDQFRSALNEND---TVFYTLG---------QGNDYVEVGGKK 68

NG1573 1 IIGPNGAGKSTLFKMIAGKEQP--------DSGEVKIGQT------------VKMSLIDQSREGLQNDK---TVFDNIA---------EGRDILQVGQFE 68

NG0840 1 .................................................................................................... 1

consensus 1 LIGPNGAGKSTLLKLLAGLEQPDSGEIMLDGQDLIGMSTQELPEMRD----RRKIGYIFQEYSLFPHMD---TVMENVAFGLV---EMQGKPKAEVEE-E 89

Walker A

110 120 130 140 150 160 170 180 190 200

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

NG0170 79 --VHEALERV------GMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFNAVDARTTYELLDVLQKC-HCGGHAIIAVLHDYEQVRAYFPNTL 170

NG2088 83 -IVNGAIEEF------HLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLDEPLNNLDMYHARSLMQILQKLTHEHKRTTVAVLHDINQAAAYADHVV 176

NG0192 79 -RVEEMLRLV------QMTKFAKRKPHQLSGGQQQRIALARSLAKRPKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIA 172

NG0445 83 -KVEELLKLV------QLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLLDEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIV 176

NG2039 79 -LALSALAEV------GLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFSSLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIA 172

NG0215 83 -RIEAMLELT------GISELAGRYPHELSGGQQQRVALARALAPDPELILLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIA 176

NG0374 91 -EALKLLEKV------GLGDKVDLYPYQLSGGQQQRVGIARALAIQPELMLFDEPTSALDPELVQDVLDAMKEL-AREGWTMVVVTHEIKFTLEVATNVV 183

NG2013 84 -QAGKLLERV------GLLDRKNAYPRELSGGQKQRIAIVRALCLNPEVILLDEITAALDPEMVREVLEVVLEL-AREGMSMLIVTHEMGFARKVADRIV 176

NG2137 84 -RVAECLEIV------GLTERAGHYPAQLSGGQKQRVGIARALAPKPQVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAA 177

NG2116 85 -LVLLKLNAV------GLRGVENLMPSELSGGMSRRVALARTIALDPEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVI 178

NG0770 85 -TAMAMLDKV------GLKHRSTHRAGELSGGERQRAAIARALVTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRF-ERVM 177

NG1922 84 -RARIAIEKV------GLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLIADEPSANLDRAYALDIMELFKTFH-EAGTTVIVAAHDETLMADYGHRIL 176

NG1439 85 -RADKLLQDL------GLASKEGNKPGELSGGQQQRVSIARALMNGGEIIFADEPTGALDTASGKNVMEIIRRLH-EAGHTVIMVTHDPGIAAN-ANRVI 176

NG1605 82 -EIEKLLADL------NIGHLRRNPAPSLSGGERRRVEIARVLAMKPRFILLDEPFAGVD.PIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAY 174

NG1399 77 -QNRGLLERV------GISQTAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEPTTGPD.PASRQMFYEVVRELNGRGATILLSTHALAELDGHADRIV 169

NG1732 84 -AAGFIPDLSDAKGRSGYDAHVGERGVKLSGGQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMME--GKTVIAIAHRLSTIAA-MDRLV 180

NG2165 84 -LQSLI-DASPL----GLHQPIGSNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLME--NRTGIIVAHRLTTVES-ADRII 175

NG1434 67 -LAAKVGLTA---------GDLNKYPTELSGGMAKRVAFLRLLLCGCDLALLDEPFVGLDRDLRDILVAMLVEKIERQGMACILVTHDRFEAARLSHEIM 157

NG0764 97 GYTAEARAAELLSGVGISEDLHNAKMAEVAPGFKLRVLLAQALFSKPDVLLLDEPTNNLD....INTIRWLEGVLNQYDSTMIIISHDRHFLNEVCTHMA 192

NG0781 91 AYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNLAQALICRADLLLLDEPTNHLD....LETVLWLENHLASLPCTQIIISHDRDFLNAATTQTI 186

NG0870 97 KDGWKLDAAVKQTLGELGLPE-NKKIGNLSGGQKKRVALAQAWVQKPDVLLLDEPTNHLD....IDAIIWLENLLKAFEGSLVVITHDRRFLDNIATRIV 191

NG1573 101 STGGGAEHELEIAADALRLPDWDAKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLD....AESVEWLEQFLVRFPGTVVAVTHDRYFLDNAAEWIL 196

NG0764 75 QVIRGTLGRLL-----FGSNDVVKKVKVLSGGEKGRMLYGKLLLLKPNVLVMDEPTNHMD....MESIESLNMALDKYNGTLIFVSHDRQFVSSLATQII 165

NG0781 66 QEIRNFLGGFD-----FVGDMALQKTEPFSGGEKARLALAMIIWQKPNLLLLDEPTNHLD....LDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFL 156

NG0870 69 KHVMSYLEDFL-----FPPARAQSPVSSLSGGERNRLLLAKLFTRPANILVLDEPTNDLD....IDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI 159

NG1573 69 IPARQYLGRFN-----FKGSDQSKIARQLSGGERGRLHLAKTLLGGGNVLLLDEPSNDLD....VETLRALEDALLEFAGSVMVISHDRWFLDRIATHIL 159

NG0840 1 ............................LSPGELQRVAFVRALLSKPKIVLLDEAAAALD........................................ 32

consensus 90 QQVRKLLERVL-----GLSDEANRKPSELSGGQRQRVALARALLQKPDILLLDEPTNALDPELAREVMEMLEELLKEYGSTVIIVTHDREFLASVADRIV 184

Signature Walker B Downstream region

...

NG0170 171 LLA 173

NG2088 177 AMK 179

NG0192 173 IMS 175

NG0445 177 VMN 179

NG2039 173 VMH 175

NG0215 177 VMK 179

NG0374 184 VMD 186

NG2013 177 FMD 179

NG2137 178 LLD 180

NG2116 179 FLA 181

NG0770 178 VMK 180

NG1922 177 RLS 179

NG1439 177 EIR 179

NG1605 175 IIS 177

NG1399 170 VMK 172

NG1732 181 VLD 183

NG2165 176 VMD 178

NG1434 158 LLS 160

NG0764 193 DLD 195

NG0781 187 ELS 189

NG0870 192 ELD 194

NG1573 197 ELD 199

NG0764 166 ELD 168

NG0781 157 LID 159

NG0870 160 VFE 162

NG1573 160 ACE 162

NG0840 32 ... 32

consensus 185 VMD 187