>1 p00 (30)

10 20 30 40 50 60 70 80

---------|---------|---------|---------|---------|---------|---------|---------|---------

HD1019 30 204 0.86 LKIYSGEMVAIVGASGSGKSTLMNIIGALDVPNSGEYFIY--------GRN------IADLSGDELAELRCRHFGFVFQR--YHLLSHL

HD860 29 204 0.86 FTINTGGLVAIVGSSGSGKSTLLHILGGLDKPTTGEVWIN--------RQS------LQQLSANQLAELRNQQLGFVYQF--HHLMADF

HD982 23 196 0.90 FHLPAGGMTYLTGHSGAGKSTLLKLIMGIEQANGGQIIFN--------GHD------ITRLDFHELPFLR-RQIGMVHQN--YRLLTDR

HD2032 22 202 0.85 LQIEKGQVVVILGPSGSGKTTLLRCLNALDMPEKGTIEFT--------GEHPLTVDFTAKEVKQQIFALR-RKSGMVFQN--YNLFPHK

HD811 23 200 0.85 VEIEKGDTAVLLGPSGAGKSTLIRTLNLMELPASGKLEIA---------QHKFNL--SAKTDAKEIALLR-KEVGMVFQQ--YHLWPHL

HD1098 26 200 0.91 LVVPKGTICGVIGASGAGKSTLIRCVNLLERPTVGNVIVD--------GQD------LTTLSSQELISAR-RSIAMIFQH--FNLLSSQ

HD572 30 199 0.91 LTINDGEFLTILGPSGCGKTTALRLIAGFEDLTEGSIILD--------GQD------VSNVSAE-----K-RPVNTVFQS--YALFPHM

HD259 41 216 0.95 LRVQKGKITAIMGPSGIGKTTLLRLIGGQIMPEIGQILFD--------GQD------ICQVSNHTLYKLR-KRMGMLFQS--GALFTDL

HD1826 19 188 1.05 LHIAAKGVTAILGRSGAGKSSLINLIAGLTTAQRGYIKLG--------EQT------LYDHEQGINLAPEKRHIGYVFQE--HRLFPHY

HD231 19 188 1.05 LQVAKAQRVAVVGESGAGKSTLLNLIAGFDLATSGQILLN--------EVD------YTFAEVA------DRPVSMLFQE--NNVFPHL

HD1820 29 198 1.05 LVVKQGDFYALLGHNGAGKSTTIGIISSLVNKTSGTVKAF--------GYD---------LDTQKI--QLKQQIGLVPQE--FN-FNQF

HD586 24 196 1.05 LNVKAGEIIGLLGPNGAGKTTTFYMVVGLVRHDQGKIRID--------DQD---------ISVLPMHDRAKYGIGYLPQE--ASIFHRL

HD1817 32 205 1.12 FQLQHGTVCALIGVNGGGKSTLFKSLMGLVKPLTGQVLLN--------QMP------IQKALKQNLVSYVPQSEEVDWQF--PVSVYDV

HD1235 28 215 0.94 LTLDEGQICGLVGESGSGKSLIAKVICGVMKDEWIVTADR--------FRFN--DIELLKLSPIQRRKLVGEQIAMIFQEPRSSLDPSK

HD1671 364 543 0.90 FTIHEGETMGLVGESGCGKSITAFSIMGLLPQTAKITGKI--------LFTDRSGKQHDLLKSANLNALRGHEISMIYQDALSALNPSM

HD315 32 217 0.90 YTVNEGEVLGIVGESGSGKSVSSLAIMGLIDFPGKVIAEQ--------LAFD--GNNLLALKAKEKQKIIGADVSMIFQDAMTSLNPSY

HD1672 42 220 0.88 LAIHAGETVGLVGQSGCGKSTLANIMIGLQ----KPTSGS--------VKFN---GLEMRYNCAEARKYFGSQVSVIFQDPSTALNPRM

HD316 49 227 0.88 FTLERGKTLAIVGESGCGKSTLGRMLTMIE----CPTEGE--------LFYN---GQNFLVNDRETAALRRQKIQIVFQNPYSSLNPRK

HD863 33 208 0.93 FNLNEGETIAIIGENGAGKSTLAKMIAGIT----KPTSGD--------MFFR---YKKLTYGDYQ---FRTKHIRMMFQDPNHTFNPNH

HD786 36 203 1.24 FSVSSGEWVQIEGHNGIGKTSLLRILAGLALPVAGEVLWN-------------------NLSIHKQRDEYYAELFYLGHY--AGIKPEL

HD1630 364 538 1.06 FTIPQGKTVALVGRSGSGKSTIASLLTRFYDVNEGQILLD--------GV---------NIEEYTLENLR-EQCSVVSQQ---VHLFND

HD1651 371 542 1.06 FEVLKGQFVAIVGKTGSGKTTIFHLLNRHYEANQGVIWLN--------QH---------KINAFAEQTLR-QNMITLSQR---VHIFNG

HD1652 373 543 1.10 FIIRPYQQIALVGPSGAGKSSLMNMLLGFL-AYQGSVKVN--------GI---------ELRDLNLAQYR-AKLAWVGQN---PQLIKG

HD1745 24 212 1.02 LHIEAGERVCLVGRNGAGKSTLMKVLAGEIQLDDGRLILEKDIVVTRLEQDPPRHIQETVFDYVAEGIAHLSQLLKEYHYISQQMLIEY

HD224 28 219 1.02 LSFFPGAKIGVLGLNGAGKSTLLRIMAGVDKEFEGEARPQPGIKIGYLPQEPKLEPQQTVREAIEEAVTEVKNALTRLDEVYALYADPD

HD1768 22 205 1.09 AIIHKGQKVGFVGKNGCGKSSLFALIKGELQAEAGEVALPKNCSMAWVNQETPA-LDISALDYVIQGNREYTTLMTQLEQ--ANLANDG

HD1338 40 195 1.26 LTIYKNSITTIVGPNGGGKSTLLKVLLKLLKPSQGQVIHQ-----------------------------PGLKIGYVPQ--KLHLDHSM

HD1745 340 496 1.08 ATIQRGDKIALVGANGCGKTTLIKLLLGQLQPTSGSIRCG-----------------------------TKLEVAYFDQY-RAELDLEK

HD224 345 501 1.08 FSIPKGAIVGIIGANGAGKSTLFRMLSGKEQPDSGSIMLG-----------------------------ETVTLASVDQF-RDAMDDKK

HD1768 333 486 1.14 LNLVPGSRIGLLGRNGAGKSTLIKLLAGKIDPLAGNIQLA-----------------------------KGVQLGYFAQH-QV---DTL

consensus FTIQKGEVVAIVGENGAGKSTLLRLLAGLVQPTSGQIMLN--------GQDN----QITKLDAQELASLRGRQIGMVFQEPRYNLFPNM

Walker A

90 100 110 120 130 140 150 160 170 180 190 200

|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|

HD1019 TAVKNV-EVPAIYAMADK--ILRNQRANALLCQLGLEKQLEN---KPAQLSGGQQQRVSIARALMNGGDIILADEPTGALDSQSSQDVLKILKDLN-RKGHTVILITHD

HD860 TALENV-MMPMLIGKQNK--REAADLAEKMLQAVGLQHRICH---RPAALSGGERQRVAIARALVNKPALVLADEPTGNLDQKTTESIFELIKQLNIEQNIAFLLVTHD

HD982 TILDNV-ALPLIIQGINQ--QIVEREARIALERVGLANKANY---RPLHLSGGEQQRVDIARAIVHRPILLLADEPTGNLDEKLSFEIFRLFEEFN-QTGTTVLVATHD

HD2032 TVLENVMEGPIFVQGKNL--AQAKLAAQQLLAKVGLADKAAL---YPNQLSGGQQQRVGIARALAIQPALMLFDEPTSALDPELVQDVLNIMKQLA-QEGWTMLVVTHE

HD811 TVMQNLIEAPIKVLGLSK--QEAITRAKQHLLRLRLNEFADR---FPLQLSGGQQQRVAIARALMMQPQVLLFDEPTAALDPEITTQVVDIIKELQ-NTGITQVVVTHE

HD1098 TVFENVALPLRL-SNTPT--EHITKKVNELLELVGLTERKDV---YPSNLSGGQKQRVAIARALASDPKVLLCDEATSALDPVTTQSILQLLKEINKRLGLTILLITHE

HD572 TIFENVAFGLRM-QKVPN--EQITPRVMEALRMVRLEDRAEQ---KPAQLSGGQQQRIAIARAVVNKPKVLLLDESLSALDYKLRKEMQNELKALQRQLGITFIFVTHD

HD259 NTFDNIAFPIREHTQLPE--ALVHKLVLMKLEAVGLRGAATL---MPAELSGGMARRAALARAIALDPELIMYDEPFTGQDPISMGVIVELIQKLNQALNLTSIVVSHD

HD1826 SVAKNL------KYGCKR--VD-NAHFLQIVKLLGIEHLLNR---YPSSLSGGEKQRVAIGRALLTKPQILLMDEPLSALDLPRKQELITYLSQLANQVDIPILYVTHS

HD231 TVEQNIGLALVPRLHLTP--QQ-QQAVKKIAGQMGIAELLLR---RADQLSGGQKQRVALARTLLQDKPILLLDEPFSALDPIRRNELQQLVLEICRQRQLTLLMVTHQ

HD1820 EKVINVLTQQAGYYGIPY--QQALIRAETWLKKLELWEKRYH---LTRELSGGMKRRLMIARALMHNPKLLILDEPTTGVDIELRRSLWDFLREIN-QQGTTIILTTHY

HD586 SVYDNLMAVLQVRKDLNN--QQRKDRAEELISEFHIEHIRDN---LGQALSGGERRRVEIARALAANPQFILLDEPFAGVDPISVIDIKKIIVHLK-QRGLGVLITDHN

HD1817 VMMGRY--GYMNFLRNPS--ALDKQKVEQAMQRVDISHLQHR---QIGELSGGQKKRVFLARALAQESQIILLDEPFTGVDVKTENAIVALLQQLR-SEGHLVLVSTHN

HD1235 NIGQQLIQTINFKGKWWQWFGWKKKKAIELLHRVGIRDHKAMMQSYPVDITEGEAQKVIIAMAVANQPRLLIADEPTHTLEPTTQLQIYRLLASMNKNLGTSILLVAND

HD1671 KIKDQMAQLISLGGKHSS--ET-------LLHWVKL-DPAKTLNRYPHELSGGQRQRVLIAMALAREPKLLIADEPTTALDVTVQAEVIKLLNELREKLGFAMVFVSHD

HD315 TVGFQIMEALKVHQGGSK--ATRRQRTIELLTMVGIPDPISRLEVYPHQLSGGMSQRVMIAMAIACNPKLLIADEPTTALDVTIQAQIIDLLLELQRKENMALILITHD

HD1672 RVLNILKDPMDIHNILLP--QEREKRVYQLLSRVGLPRSVALV--EPTRLSGGQKQRVAIARALALNPKLIVADEPTSALDVSVRAQVLNLLADLKKELNLAMLFISHD

HD316 KVGAILEESLLINTQLSA--KERKVKVLEMMEKVGLKPEFYDR--YPHMFSGGQRQRIAIARGLMLHPDIVVADEPVSALDVSVRAQVLNLMMDLQAEMGLSYVFISHD

HD863 NIGQILDAPLRLATDMNE--QERNECIFNTLKLVGMYPEYALI--PIKETSTSQKQRIALARALILSPEIIIADDTLSTLDFSVKTQLTNLMLNLQEKLGIAFIYVGQH

HD786 SAWENLRFYQKMQGL-----SLDDEALWFALDKVSLVERADL---PCSYLSAGQQRRVALAKLWLTQQKLWILDEPFTAIDNHGVGDLIMHI-ERHCSLGGMAIFTSHQ

HD1630 TIANNIAYAAKDKYSRAQIIAAAQAAHAMEFIEKLEQGLDTVIGENGASLSGGQRQRLAIARALLRNAPVLVLDEATSALDTESELAIQSALAAL--QKNKTVLVIAHR

HD1651 SLRDNLLIGDPQATDNA-LLTVLQQVGLSYLID--DHGLALWLGEGGRPLSGGEQRRLGLARVLLSHAEIVLLDEPSEGLDRVTEQQILALILQH--CTGRTLLMITHR

HD1652 SLRENILLGQQHISEQA-FKQALALSEADEFVDRL--GLDYQVQDANGGLSGGQAQRLAIARALLRHYELLLLDEPTASLDLQSEQQVLKALQNL--KGKHTTLMITHR

HD1745 SD--ELLAKLSAVQIQLE--HNNGWQFENRIQDTLKLLEL-DPDKKLCELSGGWVRRAALARALVADPDILLLDEPTNHLDVEAITWLEALLLNFK----GSIIFISHD

HD224 ADFDKLAAEQANLEAIIQ--AHDGHNLDNQLERAADALRLPDWDTKIEHLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDYE----GTVVAITHD

HD1768 NQIAHIHNHLDMIDAWTI--QARAAALLNGLGFANEQLTL-----AVKSFSGGWRMRLNLAQALLCRSDLLLLDEPTNHLDLDAVIWLEKWLTNYR----GTLLLISHD

HD1338 PI---TVKKFLSLKP-----NCSTPSIAAALTLFSIEHL-AQ--NSMQKLSGGELQRVLLARAILDRPQLLVLDEPMQGVDISGQTELYQLLTQTRTWLNCAIIMVSHD

HD1745 TVMDNVADGKQDIEV-----NGVKRHVLGYLQDFLFPPKRAM--TPVKALSGGERNRLLLAKLLLKPNNLLILDEPTNDLDV----ETLELLEELLADYQGSLLIVSHD

HD224 TVWEEVSNGQDILTI-----GNFEIPSRAYVGRFNFKGVDQQ--KRVGELSGGERGRLHLAKLLQRGGNVLLLDEPTNDLDV----ETLRALENAILEFPGCAMVISHD

HD1768 RFDESPLWHLQKLAP-----QQTEQALRNYLGGFDFKGDKVN--QLVMTFSGGEKARLVLALIVWQRPNLLLLDEPTNHLDL----DMRQALTDALTYYEGSLVVVSHD

consensus TVMENIAEGLQMIQSMSQ--QQRKQRVEZLLERVGLEEKASQLDEYPSELSGGQRQRVAIARALLQNPELLLLDEPTSALDVETQQEILQLLKELNKQLGMTILIVTHD

Signature Walker B Downstream