FN0130 1 YEELVIKAGEIVAIVGPTGSGKSRLLADIEWGAQG 35

FN0195a 1 NVNFSLNEGEIYGIVGESGSGKSTILLAIMGLLYE 35

FN0195b 1 NCSLRVRQGEIVALLGKSGSGKTTLLRIIAGLLSK 35

FN0235 1 DINIHVNENEMVAIVGNSGVGKSTLFNLIAGVLKK 35

FN0302 1 NISFEVYSGTLLSILGANGAGKTTLIKCINGILKL 35

FN0307 1 DINLKIDSGEIFTILGQNGIGKTTLLKCINGVLKW 35

FN0310 1 EFNQKFEDGEFITLLGPSGCGKTTMLRLIAGFEKP 35

FN0376 1 NLSLDIKQGEFFTLLGPSGCGKTTLLRMIAGFNSI 35

FN0399 1 GISVDIAEGETLGLVGETGAGKTTTALGIMRLITG 35

FN0400 1 NVNFAIEEGKTLGVVGESGCGKSTTGRTILRLLEA 35

FN0582 1 KLNLEVKRGEFVSILGKSGSGKSTLLNIMGLLDKI 35

FN0598 1 NINLDVKAGEIVAFVGKSGSGKTTLVNLLARFFNT 35

FN0614 1 NINLILRPNTITALVGPSGSGKTTIAYLLGRFWDI 35

FN0615 1 DINFTAKNNEITALVGKSGSGKSTIMSLIARFWDT 35

FN0660 1 DVNLKVNEGDIFGIIGLSGAGKSSLIRLINRLEEP 35

FN0669 1 NLNLDVEVGSLMALVGPNGAGKSTLIKTILKFLKQ 35

FN0695 1 KVSLEVNKGEIVGLLGPNGAGKTTTFYMITGIVKP 35

FN0770 1 ELSLNIDENKLTGIIGPNGCGKSTLAKNIIRYING 35

FN0801 1 NISTTINKGEIISIIGPSGSGKSTFLRCINKLEEP 35

FN0880 1 NLSIKIPKGAIVGLIGLNGAGKTTTLKMLTGIIIP 35

FN0882 1 DISIKVNQGQFIGVIGANGSGKSTLLKNVYRFLKY 35

FN1080 1 NFSLKIKAGTNVALVGESGVGKSTICHLIPRFYEI 35

FN1109 1 NVNIRLKKGECIGIVGESGCGKSTLARIVVGIEKK 35

FN1110 1 DVNFTLEDNQIISIVGESGSGKSTLIRAILKLLPT 35

FN1136 1 NISFNINKGEIISIIGESGAGKSTLMRCLNGLEGI 35

FN1166a 1 DVQLKLKPGTVHALMGENGAGKSTLMKCLFGIYEK 35

FN1166b 1 DVSFELYKGEILGIAGLVGSKRTEIVETIFGMRPK 35

FN1301a 1 DVNLKFTPGNCYGVIGANGAGKSTFVKILSGELEA 35

FN1301b 1 NVSFTIETGDKVVFLAKNDLVKTTLLSILAGEIEA 35

FN1348 1 DVDLNILDGDFVHIIGRSGSGKSTLLNIVAGLLSA 35

FN1352 1 EVNFQVRKGEWVAIMGSSGSGKSTIMNIIGCMDKP 35

FN1362 1 NISFEIEEGEIIGLIGESGSGKTIFTKYILGILP- 34

FN1363 1 NVSFSVKKGEIFGILGKSGAGKSTIGKILLQLLKP 35

FN1428 1 GISLEINEGEVVSIIGANGAGKTTTLQTISGLITP 35

FN1429 1 NFNLEINSGELIGLIGPNGAGKTTVFNILTGVYNA 35

FN1500 1 SFSLDIEKGKCLAIMGESGSGKSTIAKIIIGLEKQ 35

FN1501 1 NINFELKEKEIISIIGKSGSGKTMLSKMIMGLKNK 35

FN1524 1 NFSLTMQKGEIISIVGESGSGKSTVLRSIIGGLLG 35

FN1525 1 NINLSMYKGKTLGIVGESGCGKSTFLRMLMNLEKI 35

FN1648 1 DINFDIKEGEVVSLIGESGSGKTSISKIIVALLKA 35

FN1649 1 NINIEIKEKEFLGIVGESGSGKTTLLNSIISFLDE 35

FN1797 1 DINLKIEQGEFFSIIGPSGCGKTTLLRMIAGFISP 35

FN1811 1 DINLTVKAGEIHCLVGPNGGGKTSLLRCVLGQMP- 34

FN1819 1 DIDMSFKKGNLTAVVGHSGCGKSTLVSVLSGELKS 35

FN1820 1 DFSLKIKKGQLTGIMGPSGCGKSTLLKLIMRFWN- 34

FN1898a 1 NINLELKEGEILGLVGENGAGKSTLMKIIFGMEVI 35

FN1898b 1 GLDLDIKKGEILGLGGMAGQGKIAVANGVMGLFKT 35

FN1968 1 DISFKCQSGEIIGIIGPNGSGKTTLLKTINGINPI 35

FN2004 1 NLSFSIKKGEYLCIIGKNGSGKSTLAKLLAGLIFK 35

FN2005 1 DVSLEIDSHKRIAIVGHTGSGKSTLLKLIKGLLKK 35

FN2008 1 DFNLTIECGTFLTIIGSSGSGKTTILKMINGLIKA 35

FN2079 1 GLNLEINQGDFISVIGSNGAGKTTLLNTITGNIDL 35

FN2102a 1 EISFSVDEKDKIGIIGVNGAGKTTLIKLLLGLENS 35

FN2102b 1 DLNLKVYRGERIGLIGKNGTGKSTLLKIINNLEKA 35

Walker A

FN0130 1 ITSLSGGQSRALMISDTAILSTSPIVLIDEIENAGIDRKKALDLLVGNNKVLMATHDPILALMGDRRIVIKNGGIN 76

FN0195a 1 PHELSGGMRQRVFIAMGICLNP-PLLLIDEPT~TALEEESKKNILNLLKDIKRKYNTTMLIISHDFEVIEYLTEKV 74

FN0195b 1 ANELSGGQRQKVAIARALSMDP-KLLLADEIS~SMLDESGKLNIMRLLKQLQHNIGFSVLLVTHDITLAKKVADYI 74

FN0235 1 PQQLSGGMRQRVALIRTYMFKK-NIFLLDEAF~SALDAITKKELHKWYLDLKKEFNLTTLLITHDIEEAVFLSDRI 74

FN0302 1 VGELSGGEKQRVLIARALTQEP-KILILDEPI~SNLDLKFQLETMKILKNLAKEDNLIVITILHDLNFAISYSDKI 74

FN0307 1 CSELSGGQLQLVFLARALVGEP-KILILDEPE~SHLDFKNQTKILRTIVQLAKKKNITCIFNTHYPEYALRISDKS 74

FN0310 1 PSELSGGQQQRVALGRALVAQP-EILLLDEPL~SNLDAKLREEMRYEIKEITKKLKITVIYVTHDQIEAMTMSNRI 74

FN0376 1 PERLSGGQQQRVALARALVIKP-DVLLMDEPL~SNLDAKLRVEMRTAIKEIQNSIGITTVYVTHDQEEAMAVSDRI 74

FN0399 1 PHQFSGGMKQRVVIAIALACNP-KLLIADEPT~TALDVTIQAQVLDLMTDLKNKFKTSMLLITHDLGVVAQVCDKV 74

FN0400 1 PHELDGGRRQRIGIARALALNP-KFIVCDEPV~SALDVSIQAQVLNLMKDLQEKLSLTYMFITHDLSVVKYFSNDI 74

FN0582 1 PNQLSGGEKQRVAIARAMINKP-KLILADEPT~GNLDEDTGELIFSLFRKINKEHNQSIVVVTHARDLS-QVTDRQ 73

FN0598 1 GALLSGGQKQRIAIARALIKNP-EIMILDEAT~SALDSESEKLVQEALDSLMEG--RTTFVIAHRLSTI-VRADKI 71

FN0614 1 YIKLSGGEKQRISIARCLLKNS-PIVVLDEIT~AYSDIENEAKIQSAIRNLLKD--KTAIIIAHRLYTI-KDVDNI 71

FN0615 1 GSTLSGGEKQRISIARALLKNS-PIILLDEAT~ASLDADSEHEIKMAINELIKD--KTVIIIAHRLNTI-KDANKI 71

FN0660 1 PSQLSGGQKQRVSIARALANNP-DILLSDEAT~SALDPKTTKSILELIKEIQQKFSLTVLMITHQMEVVKEICNKV 74

FN0669 1 ISELSGGQQQRTFIARALVQEA-DIYLMDEPF~QGVDSTTEKSIVNILKKLKSE-GKTLIIVHHDLQTVPTYFESV 73

FN0695 1 GYSLSGGERRRIEIARTIANNP-SFILLDEPF~AGVDPIAVEDIQNIIRHLKKR-DLGILITDHNVRETLSITDKS 73

FN0770 1 VETLSGGELQKALLARALAQEA-KILLLDEPT~SALDLNNAVEFMKILKNISIKKEISVIIIIHDLNLASLFCDSL 74

FN0801 1 PSQLSGGQKQRIAIARALAMKP-EVILFDEPT~SALDPEMIKEVLDVMRDLAKEG-MTMIIVTHEMGFAKNVGNRI 73

FN0880 1 VRRLSLGERMKMELIASLIHKP-DIVFLDEPT~IGLDVITQYNIRNFLKEYCVKYGSTILLTSHNFNDIVTLCDSI 74

FN0882 1 FLSLSGGEMQRVLIARALAQNT-EILILDEPT~NHLDIKYQIQIMELVKETR----KTILAVIHDINIASSYCNYI 70

FN1080 1 GIRLSGGQKQRISIARVFLANP-PILILDEAT~SALDSITERNIQKSLDELSEG--RTTLVVAHRLTTI-RKADVI 71

FN1109 1 PHEFSGGQLQRITIARAIIIKP-KLIVCDEIT~SALDVSVQKQILELLKKLQKDLSLSYLFIGHDLAVVQDISQKI 74

FN1110 1 PFELSGGMKQRVVIAMTMAQSP-RLLLADEPT~SALHVTVQAQVIKELKRIRENFKTAIILVTHNMGVASYISDKI 74

FN1136 1 AKYLSGGEKQRVAIARSLAPNV-DLILADEPI~SSLDEKNSTQIMEIFKRINIKKNKTIILNLHNVEIAKKFSDKI 74

FN1166a 1 VADLPIAERQMIEIAKAVSYKS-KVIVMDEPT~SSLTEKEVDHLFRIIKKLKES-GVGIIYISHKMEEIKMISDEI 73

FN1166b 1 IGSLSGGNQQKVIIGRWLLTEP-EVLMLDEPT~RGIDVLAKYEIYQLMIDLAKK-DKGIIMISSEMPELLGVTDRI 73

FN1301a 1 MKELTEPEKVKVLLAQALFGEP-DVLLLDEPT~NGLDVKAISWLENFIMGLEN---STVIVVSHDRHFLNKVCTHI 71

FN1301b 1 VSVLSGGEKVRCMLSKLMLSGA-NVLLFDNPS~DHLDLESITSLNKALIKFKG----TILFGAHDHEFIQTVANRI 70

FN1348 1 PKELSGGELRRIIIARALMTDP-KILIADEPT~SDLDIEATKEVMDLLKKINEKG-TTILIVTHELDTL-KYGKKV 72

FN1352 1 PSQLSGGEQQRVCIARALINSP-EIILADEPT~GNLDEVNEKIVIEILKQLHKEG-STIVVVTHDLEVG-DVAERK 72

FN1362 1 PYELSGGEQQRIVIMGALIGEP-NFLIADEVT~TALDVETKIEIIKFFKKLQKKLKISILFITHDLSILKDFADKI 74

FN1363 1 PEELSGGQQQRVCIAGAIILSP-KLIICDEPI~ASLDLAIQVQILDLIHKINQEEGISFIFITHNLPAIYRIADRI 74

FN1428 1 AGTLSGGEQQMLAMGRALMSRP-KLLILDEPS~MGLSPLFVKEIFEVIKQLKEK-GTTILLVEQNAKMALSISDRA 73

FN1429 1 AGNLPYGEQRKLEIARAMATEP-KILLLDEPA~AGMNPKETEDLMNTIKLIRDKFGIAVLLIEHDMKLVLGICERL 74

FN1500 1 RDELSGGQLQRVCLARALSTKP-QIMIFDESL~SGLDPLVQDKILDLLYKIQKQYQLTYIFISHDFRLCYFLADRI 74

FN1501 1 PFELSGGMLQRVMVAIIIGLDA-KIIIADEVT~SALDSYNRYEMIKIFKELN-KMGKSIVLITHDYYLMKSISDRC 73

FN1524 1 PYELSGGMKQRVGIAMALTFEP-ELVLADEPT~SALDVTTQAQIVKQMMELRDEFHTGIIIVTHNMGVAAYMADKI 74

FN1525 1 PQNMSGGQKQRIGIARALSLEP-EILVCDEAT~SALDVSIQKNIIELLVKLQKERDLCIVFICHDIALVQSFAHEI 74

FN1648 1 PHELSGGQRQRVAIARAVAVKP-DLIIADEIL~TALDALTQIQILELFQKLKENKKISYLFISHDINVVKKISDRL 74

FN1649 1 PNELSGGMRQRIAIVLVLCTDI-KIFLADEPT~TSLDVVNQFRFIELLKKISKEKGLTLIYVSHDIKVLSKICERI 74

FN1797 1 VSQLSGGQQQRISIARALINKP-GVLLLDEPL~SALDAKLRQNLLIELDLIHDEVGITFIFITHDQQEALSISDRI 74

FN1811 1 LGNLSGGERQRVLLAQALFPRP-NLLILDEPL~TGIDKAGEDYFKEIVKELKSE-GMTILWIHHNLTQVKELADTV 73

FN1819 1 GKNLSGGQAQRVALARALLYDA-SVYIFDEAT~SNIDIESEEIILNIIYSLSKE--KTVIYISHRLP-AIKNADCI 71

FN1820 1 GKNFSGGERQRIGLARAFLANR-EFFLLDEPT~SNLDILNEAIILKSLADEAKD--KTVILVSHRES-TLSICNNI 71

FN1898a 1 INEMAVAYKQFTEIAREIERESTKLLVLDEPT~AVLTEDEAQILLETMKKLANK-GITIIFITHRLNEIMRVSDKV 74

FN1898b 1 VAELSGGNQQKVCVAKAFTMEP-DLLFVSEPT~RGIDVGAKQLVLETLKEYNRERNTTIVVTSSEIEELRNICDRI 74

FN1968 1 ILQLSGGERQRVLFAKILTQES-QVILLDEPT~ASLDMRHEEDLLKEVSKEKDK-DKIIILVIHNLRTAIKYCSRL 73

FN2004 1 TYQLSGGEKQRLAIASVLAMGT-EILIFDEAI~SMLDPVGKKEVLKLMKELNSQ-GKTIIHITHNRNDILEAS-EV 72

FN2005 1 TLNLSGGEKRRVALAGVLIMQP-QLLLLDEAT~VGLDLNGKEQLFKILLDWQKEENKSFLFITHDMNDVSEYAEEV 74

FN2008 1 PDELSGGQQQRVGIARALAASP-DIILMDEPF~GAVDAITRYQLQKDLKELHKKTEATIVFITHDITEALKLGTKV 74

FN2079 1 VQYLSGGQRQCLALIMATLNQP-KVLLLDEHT~AALDPKTSKIIMDKTEEIVEKFEIPTLMITHNLQDAIKYGNRL 74

FN2102a 1 IGNLSGGQNSRVALAKILLEEP-DLLVLDEPT~NHLDLTSIEWLEKILKDYN----KSIILISHDVYFLDNVVNRV 70

FN2102b 1 ISSLSGGEKARVAFMKLMLEKP-NFLILDEPT~NHLDIYSREILMDALEDYPG----TILVVSHDRNFLDTVVNKI 70

Signature Walker B Downstream region