CHU1258 NELRVIIGPNGAGKSTFMDILCGGKHLELLAGN-LSHGQKQWLEIAIVILQ--DPKLMLIDEPAAGMSDEE--HSVIVIE

CHU1259 GKVTTIMGRNGVGKTTLLKTIMS-DFRKRLGGN-LSGGQQQQLAIARVLVS--NPSLILLDEPTEGIQPSI--ITILLVE

CHU1966 EEFISIIGHSGCGKSTILSMIAGEDSFNKKASE-LSQGMKQRVGIARAFAL--KPKMLLLDEPFGMLDSLT--ITAIMIT

CHU1967 GEFIAVVGFTGSGKTTLISLIAGLPALNKTPSE-LSGGMRQRVSVARALAM--DPRILLLDEPLSALDALT--KTVLLIT

CHU2026 GVLAGIIGPNGAGKSTLIKTAMGEAFADRQISQ-LSGGQQQRVFLARALAQ--DADLYFMDEPFAGVDAST--KTIIVVH

CHU3821 NTVTALIGPSGCGKSTFLRCMNRKDKLKKSALA-LSGGQQQRLCIARALAV--EPSILLMDEPASALDPIS--YTIMIVT

CHU0218 GERVVIKGRNGSGKTTLVKILLG--YWDKTCSA-LSGGEKMRLLLCCLTILNQAPDIIVLDEPTNNLDIQN----LLVIS

CHU0313 GGSIAFVGSTGAGKSSIINLLNRDYEVMERGST-LSSGQRQIISFIRVMVY--DPKILILDEATSSIDSET--RTSIIIA

CHU0387 GERFGLFGPNGAGKSTLISLMTGTDVKHKAVKT-FSGGMKRRVNLAIGVIH--QPRILFLDEPTVGVDIQT--MTLVYTS

CHU0463 GETIGLVGESGCGKTTLGRSILRRKQFNRYPHE-FSGGQRQRICIARALALN--PKFIICDESVSALDVSV--FTYIFIS

CHU0485 NSVLGLLGPNGAGKTTLLRLITQEHLQSQVLET-LSKGQQQKIQFISAVVH--QPELLILDEPFSGFDPAN--TTIIYST

CHU0506 GKLIALVGPSGGGKSTLVDLIPRQTSIGERGSK-LSGGQRQRLSIARAVLK--NPDILILDEATSALDNES--RTSIVIA

CHU0800 NEKLGIIGKNGSGKTTLLQLINRSRQY-EFIEK-LSGGEKKRLHLLTILVR--QPNFLILDEPTNDMDLST----LIIVS

CHU0848 GDAVGIIGKNGAGKSTLLKILSQVEKYIDTPVKRYSSGMYVRLAFAVAAHL--EPEILIVDEVLAVGDAEF--RTVLFVS

CHU0977 GEVHAIMGPNGSGKSTLASVLAGESLLKRALNEGFSGGEKKRNEIFQMAML--EPSLAILDETDSGLDIDA--NATIVVT

CHU1035 CSTLAITGANGSGKSTLLQVLSS-----KEIRN-FSSGMKQRVKLGLALLS--DTPLLFLDEPTSNLDEQG---KTIIIA

CHU1545 GRITGFLGPNGAGKSTTMKVATCGVEQYKKLGA-LSKGYRQRAGLAQALIH--DPQVLILDEPTTGLDPNQ--KTVIFST

CHU1763 GEKVAFVGKNGEGKSTLIKAIMSEASA-KKVKV-LSGGEKTRLAMIKLLLE--PVNLLILDEPTNHLDIKT----IILVS

CHU1770 GEITGIVGENGNGKTTLLRILARLSNFAHLGWKELSSGYKLRFELAKMLVWS--PRILVLDEPLANLDINANPVSVILSS

CHU1843 GDVIFITGDNGSGKSTLLKAIARITHLLNRNAKELSGGELQLCWLVQSFLA--APPVLLLDEPTQYLDAKN--FICICVT

CHU1845 GGIVGIIGPNGAGKTTLFKLITGSDQE-KKIGV-LSGGERNRVHLAMTLKT--GANLLLLDEPTNDLDVNT----AVIIS

CHU2049 GQTIAFVGPSGSGKTTLVKLLVGDTVIGEGGVK-VSGGEKQRLSIARALLR--QPDILVFDEATSSLDSIT--HITILIA

CHU2145 HVLVADLG-GGTTDFTFIELDPHKNLITLTENN-LGYSIFQSIEKTKVDLSIQSSSSFSYHKADIHIDEPIN----NYLE

CHU2216 GDKIALIGANGKGKSTVLRILTGDDAF-KKIKV-LSGGEKSRVALAKTLIS--DANYLLLDEPTNHLDMVS----LIVIS

CHU2233 GEVVSIVGASGAGKSTLLHILGSDHRFTHYPNQ-LSGGEQQRVAVARSLIN--DPLIVFADEPSGNLDTHN--QTFVIVT

CHU2416 GKKLAIVGESGSGKTTLLKLLAGQQLLDRKSYQ-LSGGQQQRAAIAAAFAA--SPQVMLMDEPFSNLDVLL--TSLIFVT

CHU2448 GQTLAIIGNTGSGKSTIASLLCRDTILGERGIT-LSGGQKQRLSLARAFIR--NPRLFILDDCLSAVDTKT--RSTIIIS

CHU2644 GELLLIIGPSGSGKTTLLSLIGCLDRKKNLPKQ-LSGGQQQRIAIARALVT--NPKIILCDEPTASLDKDS--GKAVVVV

CHU2985 GENVVVLGKSGTGKSVLIKILVGIDKLNQMPSD-LSGGQRKRIGIARTLIL--KPKIMLYDEPTSGLDPIT--TSSIIIT

CHU3458 ADKVSILSKEPLSISALFDVLMGEESL-KKCTV-LSGGEKVRCMLSKMMME--SANVLIMDDPTNHLDLES----MLFYS

CHU3530 GTVTALVGTSGSGKSTIAGLVATDTLIGERGVK-LSGGQRQRIAIARAILA--DPKIIILDEATSNLDTQS--RTTFVIA

CHU3635 GEHWALLGHNGSGKSTLLSLINAVIHLKQRAFYKTSLGQQRLLLLARALVK--NPPVLILDEPCQGLDKEQK--TLIYVS

CHU3669 GEIVGLLGPNGAGKTTSFYMIVGTHVRKNLGKV-LSGGERRRTEIARALAV--DPKFVLLDEPFAGVDPIA--IGILITD

Walker A Signature Walker B

CHU1258 HDMGFVKQIAQNLVTVLVRGQ

CHU1259 QKIDFAKQITDYYYFMDR-GR

CHU1966 HDVDEAILLSDRVVMMTS-GP

CHU1967 NDVDEGIYLADRIIPLKP-GP

CHU2026 HDLNSADEYFDWILLLNT--R

CHU3821 HNMQQASRTSDKTAFFYM-GE

CHU0218 HDIYFMEQIRVERTIELI---

CHU0313 HRLST-IQHVDTIFVLEK-GE

CHU0387 HQLAEAESLCNRIALIDN-GQ

CHU0463 HDLSVVKFMSDRIIVMNK-GK

CHU0485 HRMDTVEDVCTELVLINQ-AK

CHU0506 HRLST-IRHADMILVLDK-GQ

CHU0800 HDRYFMDKLVDHLLVFEG-EG

CHU0848 HNMGAVAELCRSGIVLSN-GN

CHU0977 HYQRLLDYIVPDFVHVLYKGR

CHU1035 SNIPEEYAFAQKVLKISD--Y

CHU1545 HIMQEVQALCDQVIIINR-GK

CHU1763 HDRDFLNGLATKVFEFG--NK

CHU1770 QQLHEVEHVADNIIFLRN-GR

CHU1843 HDLPFKKEDYGQVLNLSN---

CHU1845 HDRWFLDRVATHILAFEG-DS

CHU2049 HRLST-IMHADTIFVLEK-GN

CHU2145 SFLSKHAIVPESIDSLFLTGG

CHU2216 HDRHFVSKVANKIWYIE--DQ

CHU2233 HNEKLADLS-DRKVVMQD-GV

CHU2416 HQTTDALALADTILVMRN-GK

CHU2448 HRVSS-VKLADKIIVLED-GQ

CHU2644 THDPRLKEYAHRIVEVKN-GT

CHU2985 HDLTCAKETCDRVAMLLD-GT

CHU3458 HDYQFINTIATRIIELGP-NG

CHU3530 HRLST-IRQANQILVIEN-GK

CHU3635 HYASDMPSCIDHVLMLEN-GR

CHU3669 HNVNETLSITDRAYLLFE-GK

Downstream region