Gene ID: NG1665
DNA Molecule Name:
1
Genbank ID:
Gene Name:
ilvE
Definition:
branched-chain amino acid aminotranferase
Gene Start:
1619120
Gene Stop:
1620115
Gene Length:
996
Molecular Weight*:
36116
pI*:
5.90
Net Charge*:
-5.29
EC:
2.6.1.42
Functional Class:
Amino acid biosynthesis; Branched chain amino acid family
Pathway: pathway table
Pantothenate and CoA biosynthesis
Valine, leucine and isoleucine biosynthesis
Valine, leucine and isoleucine degradation
Secondary Evidence:
Cox,J.L., Cox,B.J., Fidanza,V. and Calhoun,D.H.
The complete nucleotide sequence of the ilvGMEDA cluster of Escherichia coli K-12.
Gene 56 (2-3): 185-198 (1987)
[Medline: 88056322].
Flint,D.H., Emptage,M.H., Finnegan,M.G., Fu,W. and Johnson,M.K. The role and properties of the iron-sulfur cluster in Escherichia coli dihydroxy-acid dehydratase.
J. Biol. Chem. 268 (20): 14732-14742 (1993)
[Medline: 93315441].
Brown,O.R., Smyk-Randall,E., Draczynska-Lusiak,B. and Fee,J.A. Dihydroxy-acid dehydratase, a [4Fe-4S] cluster-containing enzyme in Escherichia coli: effects of intracellular superoxide dismutase on its inactivation by oxidant stress.
Arch. Biochem. Biophys. 319 (1): 10-22 (1995)
[Medline: 95289710].
Kuramitsu,S., Ogawa,T., Ogawa,H. and Kagamiyama,H.
Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence.
J. Biochem. 97 (4): 993-999 (1985)
[Medline: 85289113].
Comment:
See also ilvA, NG0444; ilvC, NG1233; ilvH, NG1235; ilvI, NG1236; ilvE, NG1665.
Oklahoma ID: NGO.1665
Blast Summary: PSI-Blast Search
Residues 1-332 in NG1665 have 98% similarity to residues 1-332 in AL162758, N. meningitidis Z2491 putative branched-chain amino acid aminotransferase.
Residues 9-328 in NG1665 have 38% similarity to residues 28-357 in
AE004019, X. fastidiosa branched-chain amino acid aminotransferase.
Residues 9-328 in NG1665 have 36% similarity to residues 29-358 in
AL031124, S. coelicolor A3(2) branched-chain amino acid aminotransferase.
COGS Summary: COGS Search
BeTs to 12 clades of COG0115
COG name: Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase
Functional Class: E,H
The phylogenetic pattern of COG0115 is amt-YQvCEBRhUJ------x
Number of proteins in this genome belonging to this COG is 1
Blocks Summary: Blocks Search
***** IPB001544 (Aminotransferases class-IV) with a combined E-value of 7.5e-38.
IPB001544A 47-58
IPB001544B 69-83
IPB001544C 119-133
IPB001544D 174-184
IPB001544E 236-251
IPB001544F 275-288
ProDom Summary: Protein Domain Search
Residues 36-288 are 42% similar to a (AMINOTRANSFERASE ACID TRANSFERASE PYRIDOXAL PHOSPHATE) protein domain (PD001961) which is seen in ILVE_BACSU.
Paralogs: Local Blast Search
Pfam Summary: Pfam Search
Residues 29 to 314 (E-value = 2.8e-70) place NG1665 in the Aminotran_4 family which is described as Aminotransferase class IV (PF01063)
Structural Feature(s):
| Feature Type | Start | Stop |
| transmembrane |
PDB Hit:
pdb|1EKF|1EKF-A CRYSTALLOGRAPHIC STRUCTURE OF HUMAN BRANCHED 162.0 6e-41
pdb|1A3G|1A3G-A BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE FROM 100.0 4e-22
pdb|1I1K|1I1K-A CRYSTAL STRUCTURE OF ESCHELICHIA COLI 100.0 4e-22
pdb|1DAA|1DAA-A CRYS
Gene Protein Sequence:
MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGAHALHYG
SECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDAL
VELVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPV
GDYFKVGSPVKILVETEHIRCAPHMGRVKCGGNYASAMHWLLKAKAEYGA
NQVLFCPNGDVQETGASNFILINGDEIITKPLTDEFLHGVTRDSVLTVAK
DLGYTVSERNFTVDELKAAVENGAEAILTGTAAVISPVTSFVIGGKEIEV
KNQERGYAIRKAITDIQYGLAEDKYGWLVEVC
Gene Nucleotide Sequence: Sequence Viewer
ATGAGCAGACCCGTACCCGCCGTATTCGGCAGCGTTTTTCACAGTCAAAT
GCCCGTCCTCGCCTACCGCGAAGGCAAATGGCAGCCGACCGAATGGCAAT
CTTCCCAAGACCTTACCCTTGCACCCGGCGCGCATGCCCTGCATTACGGC
AGCGAATGCTTTGAAGGTTTGAAGGCCTTCCGTCAGGCAGACGGTAAAAT
CGTGCTGTTCCGTCCGACCGCCAATATCGCGCGTATGCGGCAAAGTGCGG
ACATTTTGCACCTGCCGCGCCCCGAAACCCAAGCTTATCTCGATGCTTTG
GTCGAACTGGTCAAACGTGCCGCCGACGAAATTCCCGATGCGCCTGCCGC
GCTGTACCTGCGCCCGACCTTAATCGGTACCGATCCCGTTATCGGCAAGG
CCGGTTCTCCTTCCGAAACCGCCCTGCTGTATATTTTGGCTTCCCCCGTC
GGCGACTATTTCAAAGTCGGCTCGCCCGTGAAAATCTTGGTGGAAACCGA
ACACATCCGCTGCGCCCCGCATATGGGCCGCGTCAAATGCGGCGGCAACT
ACGCTTCCGCCATGCACTGGTTGCTGAAGGCGAAAGCCGAATACGGCGCA
AATCAAGTCCTGTTCTGCCCGAACGGCGACGTTCAGGAAACCGGCGCATC
CAACTTTATCCTGATTAACGGCGATGAAATCATTACCAAACCGTTGACAG
ACGAATTCCTGCACGGCGTTACCCGCGATTCCGTGCTGACTGTCGCCAAA
GATTTGGGCTATACCGTCAGCGAACGTAATTTCACGGTTGACGAACTGAA
AGCTGCTGTGGAAAACGGTGCGGAAGCGATTTTGACCGGTACGGCCGCCG
TCATCTCGCCCGTTACTTCCTTCGTCATCGGCGGCAAAGAAATCGAAGTG
AAAAACCAAGAACGCGGCTATGCCATCCGTAAGGCGATTACCGACATCCA
GTACGGTTTGGCGGAAGACAAATACGGCTGGCTGGTCGAAGTGTGC
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