Basic Search | Intermediate Search | Advanced SQL Search | Gene Image Map |  Home

Neisseria gonorrhoeae Search Results

Record: 1 of 1  
MiniMap IGR0672 IGR0673 IGR0671.2 IGR0670 IGR0671.3 IGR0671 IGR0671.1 NG0811 bioB, - NG0813 cysI, - NG0807 NG0810 cybP, - NG0805 NG0812 cysN,nodP, - NG0806 cysI, - NG0808 dad,ilvD, - NG0809 NG0811 bioB, - NG0813 cysI, - NG0807 NG0810 cybP, - NG0805 NG0812 cysN,nodP, - NG0806 cysI, - NG0808 dad,ilvD, - NG0809 Type: tandem, Name:  - 35 NG0811 bioB, - NG0813 cysI, - NG0807 NG0810 cybP, - NG0805 NG0812 cysN,nodP, - NG0806 cysI, - NG0808 dad,ilvD, - NG0809
* Calculated from Protein Sequence

Gene ID: NG0808

DNA Molecule Name:
1  

Genbank ID:


Gene Name:
cysI  

Definition:
NADPH-sulfite reductase hemoprotein beta component

Gene Start:
801186

Gene Stop:
802556

Gene Length:
1371

Molecular Weight*:
50231

pI*:
7.50

Net Charge*:
2.16

EC:
1.8.1.2  1.8.7.1  

Functional Class:
Central intermediary metabolism; Sulfur metabolism  

Pathway: pathway table
Selenoamino acid metabolism
Sulfur metabolism

Secondary Evidence:
Gruez A, Pignol D, Zeghouf M, Covès J, Fontecave M, Ferrer JL, Fontecilla-Camps JC.
Four crystal structures of the 60 kDa flavoprotein monomer of the sulfite reductase indicate a disordered
flavodoxin-like module.
J Mol Biol. 2000 May;299(1):199-212.
PMID: 10860732

Willson PJ, Gerlach GF, Klashinsky S, Potter AA.
Cloning and characterization of the gene coding for NADPH-sulfite reductase hemoprotein from Actinobacillus
pleuropneumoniae and use of the protein product as a vaccine.
Can J Vet Res. 2001 Oct;65(4):206-12.
PMID: 11768126

Ostrowski,J., Wu,J.Y., Rueger,D.C., Miller,B.E., Siegel,L.M. and
Kredich,N.M.
Characterization of the cysJIH regions of Salmonella typhimurium
and Escherichia coli B. DNA sequences of cysI and cysH and a model
or the siroheme-Fe4S4 active center of sulfite reductase
hemoprotein based on amino acid homology with spinach nitrite
reductase
J. Biol. Chem. 264 (26), 15726-15737 (1989)

Crane,B.R., Siegel,L.M. and Getzoff,E.D.
Sulfite reductase structure at 1.6 A: evolution and catalysis for
reduction of inorganic anions
Science 270 (5233), 59-67 (1995)
MEDLINE: 96008578

Crane,B.R., Siegel,L.M. and Getzoff,E.D.
Structures of the siroheme- and Fe4S4-containing active center of
sulfite reductase in different states of oxidation: heme activation via reduction-gated exogenous ligand
exchange
Biochemistry 36 (40), 12101-12119 (1997)
MEDLINE: 97461529

Comment:


Blast Summary:  PSI-Blast Search
Residues 3-447 are 65% similar to residues residues 135-570 in U29579, a sulfite reductase (NADPH) hemoprotein alpha subunit Escherichia coli.

Residues 3-447 are 65% similar to residues 135-570 in RDECSH sulfite reductase (NADPH) (EC 1.8.1.2) hemoprotein from Escherichia coli.

COGS Summary:  COGS Search
BeTs to 7 clades of COG0155
COG name: Sulfite reductase hemoprotein beta-component
Functional Class:  P
The phylogenetic pattern of COG0155 is a---yq-CebR----------
Number of proteins in this genome belonging to this COG is 2

Blocks Summary:  Blocks Search
***** IPB000660 (Nitrite and sulfite reductase) with a combined E-value of 3.9e-16.
    IPB000660A    77-90
    IPB000660B    354-372


ProDom Summary:  Protein Domain Search


Paralogs:  Local Blast Search


NG0808 has no significant similarity (blastp p-value < 1e-3) to any other gene in this genome.


Pfam Summary:  Pfam Search
Residues 40 to 207 (E-value = 2.7e-42) place NG0808 in the NIR_SIR family which is described as Nitrite and sulphite reductase 4Fe-4S domain (PF01077)
Residues 223 to 294 (E-value = 5.1e-16) place NG0808 in the NIR_SIR_ferr family which is described as Nitrite/Sulfite reductase ferredoxin-like half domain (PF03460)

PDB Hit:
pdb|1AOP|1AOP SULFITE REDUCTASE STRUCTURE AT 1.6 ANGSTROM 615.0 0e+00

Gene Protein Sequence:
MPRLLHSLGLDSIATAADMNRNVLCTSNPIESELHRQAYEYAKKISEHLL
PRTRGYLDMWVDGKKVQSSDDFLQEGEPILGKTYLPRKFKTAVVIPPLND
VDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSTEHGNGNTKTYPNISLEL
GFVPPEHALKAAEAVVTTQRNFGNRSHRKNDRTRYTIQNMGLDNFRTEAE
RRMGMPFKPIRPFKFTGRGDRIGRVKGIDGNWHLTLFIESGRLVDEGGKQ
LLTGVLEIAKIHKGGFRITANQNLIVANVPEGGKARIEQLARSYGLIRDG
VSKLRENAMSCVSFPTCPLAMAEAERVLPDFIGELDKIMAEHGTSDDYIV
TRITGCPNGCGRAMLAEIGLVGKAVGRYNLHIGGDREGVRIPRLYKENIT
LPEILSELEDLIGKWAAGRDTDEGFGDFAIRTGIVKPVLDAPVDFWDASK
AVPIARA

Gene Nucleotide Sequence:  Sequence Viewer
ATGCCCCGCCTCTTGCACAGCCTGGGTTTGGATTCCATCGCTACGGCGGC
GGATATGAACCGCAATGTGCTTTGCACCTCCAACCCGATCGAGTCCGAGC
TGCACCGGCAGGCTTACGAATACGCGAAAAAGATTTCCGAACACCTGCTG
CCGCGCACGCGCGGTTATCTGGATATGTGGGTGGACGGCAAAAAAGTTCA
AAGTTCCGACGACTTCCTTCAAGAAGGCGAGCCGATTTTGGGCAAAACCT
ATCTGCCGCGAAAATTCAAAACCGCAGTCGTCATCCCGCCCTTGAACGAT
GTGGACTGCTACGGTAACGATTTGGATTTCGTCGCCGTTTCAGACGGCAA
CGGACAGCTTGCCGGCTTCAATGTTTTGGCGGGCGGCGGGCTTTCGACGG
AACACGGCAACGGCAACACCAAAACCTATCCGAACATTTCACTGGAACTG
GGTTTCGTGCCTCCGGAACACGCACTGAAGGCCGCCGAAGCGGTGGTAAC
CACGCAGCGCAACTTCGGCAACCGCAGCCACCGCAAAAACGACCGCACCC
GCTACACCATTCAAAATATGGGCTTGGACAACTTCCGCACGGAAGCCGAA
CGCCGTATGGGTATGCCGTTCAAACCCATACGGCCGTTCAAGTTTACCGG
GCGCGGCGACCGCATCGGCCGGGTGAAAGGCATAGACGGCAACTGGCATT
TGACCCTTTTCATCGAAAGCGGGCGTTTGGTTGACGAAGGCGGGAAACAG
CTTCTGACCGGCGTGTTGGAAATCGCCAAAATCCACAAAGGCGGCTTCCG
CATCACCGCCAACCAAAACCTCATCGTGGCAAATGTGCCCGAAGGAGGCA
AAGCCCGGATCGAACAGCTCGCCCGCTCATACGGGCTGATACGCGACGGT
GTCAGCAAGCTGCGCGAAAATGCGATGTCGTGCGTTTCCTTCCCGACCTG
CCCGCTGGCAATGGCGGAAGCCGAACGCGTGTTGCCGGACTTCATCGGCG
AGCTGGATAAGATTATGGCGGAACACGGCACGTCGGACGACTACATCGTT
ACCCGCATTACCGGCTGCCCGAACGGCTGCGGACGGGCGATGTTGGCGGA
AATCGGACTGGTCGGCAAAGCCGTGGGACGCTACAACCTCCACATCGGCG
GCGACCGCGAAGGCGTACGCATCCCCCGCCTCTACAAAGAAAACATCACC
CTGCCGGAAATCCTTTCCGAATTGGAAGACCTGATCGGCAAATGGGCGGC
GGGACGCGATACCGACGAAGGTTTCGGCGATTTCGCCATACGGACGGGCA
TCGTCAAACCCGTGTTGGATGCGCCCGTCGATTTTTGGGACGCATCCAAA
GCCGTCCCGATTGCGCGCGCC


Los Alamos National Laboratory     
Operated by the University of California for the National Nuclear Security Administration,
of the US Department of Energy.     Copyright © 2001 UC | Disclaimer/Privacy