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Mycoplasma genitalium Search Results

Record: 1 of 1  
MiniMap IGR309 IGR307 IGR308 IGR304 IGR303 IGR302 IGR306 IGR305 atpE, - MG404 atpF, - MG403 MG409 atpB, - MG405 MG412 eno, - MG407 atpA, - MG401 pstA, - MG411 pmsR, - MG408 atpE, - MG404 atpF, - MG403 MG409 atpB, - MG405 MG412 eno, - MG407 atpA, - MG401 pstA, - MG411 pmsR, - MG408 atpE, - MG404 atpF, - MG403 MG409 atpB, - MG405 MG412 eno, - MG407 atpA, - MG401 pstA, - MG411 MG406 atpH, - MG402 pstB, - MG410 MG406 atpH, - MG402 pstB, - MG410 pmsR, - MG408
* Calculated from Protein Sequence

Gene ID: MG407

DNA Molecule Name:
1  

Genbank ID:


Gene Name:
eno  

Definition:
enolase

Gene Start:
510836

Gene Stop:
509463

Gene Length:
1374

Molecular Weight*:
50008

pI*:
7.14

Net Charge*:
0.38

EC:
4.2.1.11  

Functional Class:
Energy metabolism; Glycolysis and Gluconeogenesis  

Pathway: pathway table
Amino Acid Metabolism; Phenylalanine, tyrosine and tryptophan biosynthesis
Carbohydrate Metabolism; Glycolysis / Gluconeogenesis

Comment:
Taken from Prosite PDOC00148: Enolase is a glycolytic enzyme that catalyzes the dehydration of 2-phospho-D-glycerate to phosphoenolpyruvate. It is a dimeric enzyme tht requires magnesium (Mg) both for catalysis and stabilizing the dimer.

Reference: J. Biol. Chem. 1989. (264):3685-3693.

In B.subtilis, this protein is found in an operon that contains the following proteins

Blast Summary:  PSI-Blast Search
Hits in gapped BLAST to Enolase (2-phosphoglycerate dehydratase)
sequences, e.g. residues 11-454 are 75% similar to ENO_MYCPN.
MG407 is similar to C.trachomatis enolase sequence CT587.
It shows no significant similarities to T.pallidum sequences.

COGS Summary:  COGS Search
The phylogenetic pattern of COG0148 is ehgpcmy.
COG name: Enolase.
Functional Class: C
BeTs to e,g,c,m,y.

Blocks Summary:  Blocks Search
Residues 42-437 represent 8 matches to blocks BL00164A-G
(Enolase proteins).

ProDom Summary:  Protein Domain Search
Residues 15-436 constitute a defined domain of ENO_MYCGE.

Paralogs:  Local Blast Search
No evidence of paralogs in M.genitalium.

Pfam Summary:  Pfam Search
Residues 11 to 142 (E-value = 2.2e-56) place MG407 in the Enolase_N family which is described as Enolase, N-terminal domain (PF03952)
Residues 153 to 451 (E-value = 3.3e-149) place MG407 in the Enolase_C family which is described as Enolase, C-terminal TIM barrel domain (PF00113)

Structural Feature(s):
Feature Type  Start  Stop
non-globular  
65  
168

PDB Hit:
gi|1065025|pdb|1EBG|A Chain A, Enolase (E.C.4.2.1.11) (2-Phospho-D-Glycerate Hydrolase) (Apo Form)

Gene Protein Sequence:
MGSSNLNINSKITDIFAYQVFDSRGVPTVACVVKLASGHVGEAMVPSGAS
TGEKEAIELRDNDPKNYFGKGVNEAVDNVNKVIAPKLIGLNAFDQLTVDQ
AMIKLDNTPNKAKLGANAILSVSLAVSKAAAKAQNSSLFQYISNKLIGLN
TTNFVLPVPMLNVINGGAHADNYIDFQEFMIMPLGAKKMHEALKMASETF
HALQNLLKKRGLNTNKGDEGGFAPNLKLAEDALDIMVEAIKLAGYKPWDD
IAIAIDVAASEFYDEDKKLYVFKKGIKANILNAKDWSLTSKEMIAYLEKL
TKKYPIISIEDGLSENDWEGMNQLTKTIGSHIQIVGDDTYCTNAELAKKG
VAQNTTNSILIKLNQIGSISETIQTIEVAKKANWSQVISHRSGETEDTTI
ADLAVAAQTGQIKTGSMSRSERIAKYNRLLYIEIELGDKGKYLGWNTFTN
IKPKNFNI

Gene Nucleotide Sequence:  Sequence Viewer
ATGGGAAGTTCAAATCTAAACATCAATTCAAAAATAACCGATATTTTTGC
TTATCAAGTTTTTGATTCTCGGGGTGTTCCAACAGTAGCTTGTGTTGTTA
AATTGGCATCTGGTCATGTAGGTGAAGCGATGGTTCCATCAGGTGCTTCT
ACAGGTGAGAAAGAAGCAATTGAATTACGTGATAATGATCCAAAAAATTA
TTTTGGTAAAGGCGTTAACGAAGCCGTTGATAACGTTAATAAAGTTATTG
CCCCTAAGCTTATTGGCTTAAATGCATTTGATCAATTAACAGTGGATCAA
GCAATGATTAAACTAGACAATACTCCCAACAAAGCAAAATTAGGAGCAAA
TGCTATATTATCTGTTTCACTTGCAGTATCAAAAGCAGCAGCAAAAGCAC
AAAACAGCTCATTATTTCAATACATTTCAAATAAATTAATTGGATTAAAT
ACAACAAATTTTGTTTTACCTGTGCCAATGTTAAATGTAATTAATGGTGG
TGCTCATGCTGATAACTATATTGATTTTCAAGAGTTCATGATCATGCCTT
TAGGTGCTAAAAAGATGCATGAAGCTTTAAAAATGGCTAGTGAAACTTTT
CATGCTTTACAAAATCTTTTAAAAAAGCGTGGATTAAACACAAATAAAGG
AGATGAAGGTGGATTTGCGCCTAACTTAAAACTTGCAGAAGATGCACTTG
ACATCATGGTTGAAGCCATTAAATTAGCTGGATATAAGCCTTGAGATGAT
ATTGCTATTGCCATTGATGTTGCTGCTAGTGAGTTTTATGACGAAGATAA
AAAACTTTATGTTTTCAAGAAAGGAATAAAAGCTAATATCCTTAATGCAA
AGGATTGGAGTTTAACAAGCAAAGAAATGATTGCTTACTTAGAAAAATTA
ACAAAAAAATATCCAATTATTTCAATAGAAGATGGTTTGAGTGAAAATGA
TTGAGAAGGGATGAACCAATTAACTAAAACCATAGGTAGCCATATTCAAA
TTGTTGGTGATGACACTTACTGTACTAATGCAGAACTTGCTAAAAAAGGT
GTTGCACAAAATACAACAAACTCGATATTGATTAAATTAAATCAAATTGG
TTCTATTAGTGAAACGATTCAAACAATTGAAGTTGCAAAAAAAGCTAACT
GGAGTCAAGTAATTTCACATCGCAGTGGTGAAACAGAAGATACAACTATT
GCTGATTTGGCAGTTGCTGCCCAAACTGGTCAAATTAAAACCGGTTCAAT
GTCACGCTCAGAAAGAATAGCTAAATACAATCGTTTGTTGTACATAGAAA
TTGAACTTGGTGATAAAGGAAAATACTTAGGTTGAAATACCTTTACAAAC
ATTAAACCTAAAAACTTTAACATC


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