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Mycoplasma genitalium Search Results

Record: 1 of 1  
MiniMap IGR6 IGR5 IGR4 IGR8 IGR3 IGR7 rimK, - MG012 MG011 dnaE_1, - MG010 holB,dnaH_1, - MG007 MG009 tdhF, - MG008 gyrB, - MG003 gyrA, - MG004 rimK, - MG012 MG011 dnaE_1, - MG010 holB,dnaH_1, - MG007 MG009 tdhF, - MG008 gyrB, - MG003 gyrA, - MG004 Type: inverted, Name:  - 59 Type: inverted, Name:  - 59 Type: inverted, Name:  - 65 rimK, - MG012 MG011 dnaE_1, - MG010 holB,dnaH_1, - MG007 MG009 tdhF, - MG008 gyrB, - MG003 gyrA, - MG004 tmk, - MG006 serS, - MG005 tmk, - MG006 serS, - MG005
* Calculated from Protein Sequence

Gene ID: MG006

DNA Molecule Name:
1  

Genbank ID:
1045674

Gene Name:
tmk  

Definition:
thymidylate kinase

Gene Start:
8552

Gene Stop:
9181

Gene Length:
630

Molecular Weight*:
24233

pI*:
9.15

Net Charge*:
5.06

EC:
2.7.4.9  

Functional Class:
Purines, pyrimidines, nucleosides, and nucleotides; Nucleotide and nucleoside interconversions  

Pathway: pathway table
Nucleotide Metabolism; Pyrimidine metabolism

Secondary Evidence:
Reynes JP, Tiraby M, Baron M, Drocourt D, Tiraby G. 1996. Escherichia coli thymidylate kinase: molecular cloning,
nucleotide sequence, and genetic organization of the
corresponding tmk locus. J Bacteriol 178(10):2804-1. Medline: 8631667.

Binkley JP, Kuempel PL. 1986. Genetic mapping in Escherichia coli of tmk, the locus for dTMP kinase. J Bacteriol 168(3):1457-8. Medline: 3023302.

Comment:
MG006 has two N-gylcosylation sites (58-61, NETM, and 130-133, NFTF). See Prosite PDOC00001. There are also four protein kinase C phosphorylation sites (94-96, SDR; 108-110, SKK; 115-117, TVK;
167-169, TVR). See Prosite PDOC00005.

Blast Summary:  PSI-Blast Search
Hits in gapped BLAST to thymidylate kinase sequences, e.g. residues 1-208 are 62% similar to KTHY_MYCPN (sp|P75106).
MG006 is similar to T. pallidum TP0354, a predicted thymidylate
kinase. It is also similar to other predicted thymidylate kinases from C. trachomatis (CT188), Bacillus subtilis (sp|P37537), Aquifex aeolicus (sp|O67099) among others.

COGS Summary:  COGS Search
The phylogenetic pattern of COG0125 is ehgpcmyu.
COG name: Thymidylate kinase.
Functional Class: F
BeTs to -hg-cmyu.
Number of proteins in this genome belonging to this COG is 1.

Blocks Summary:  Blocks Search
None.

ProDom Summary:  Protein Domain Search
Residues 3-114, 4-151, 152-210 constitute defined domains of
Y006_MYCGE. In addition to Y006_MYCGE it also has hits to KTHY_YEAST.

Paralogs:  Local Blast Search
There is no evidence of paralogs in M. genitalium.

Pfam Summary:  Pfam Search
Residues 9 to 197 (E-value = 1.5e-83) place MG006 in the Thymidylate_kin family which is described as Thymidylate kinase (PF02223)

PDB Hit:
gi|3318768|pdb|2TMK|A Chain A, Yeast Thymidylate Kinase Complexed With 3'-Azido-3'-Deoxythymidine Monophosphate (Azt-Mp) Transferase (Phosphotransferase), Kinase, Thymidine Activation Pathway, Enzyme, Azt Mol_id: 1; Molecule: Thymidylate Kinase; Chain: A.

Gene Protein Sequence:
MNKGVFVVIEGVDGAGKTALIEGFKKLYPTKFLNYQLTYTREPGGTLLAE
KIRQLLLNETMEPLTEAYLFAAARTEHISKLIKPAIEKEQLVISDRFVFS
SFAYQGLSKKIGIDTVKQINHHALRNMMPNFTFILDCNFKEALQRMQKRG
NDNLLDEFIKGKNDFDTVRSYYLSLVDKKNCFLINGDNKQEHLEKFIELL
TRCLQQPTHY

Gene Nucleotide Sequence:  Sequence Viewer
ATGAATAAAGGTGTTTTTGTTGTTATTGAAGGAGTTGATGGAGCGGGCAA
AACTGCTTTAATTGAAGGTTTTAAAAAACTTTATCCAACTAAGTTTTTGA
ACTATCAACTTACTTATACTAGAGAACCTGGTGGTACTTTGTTAGCTGAA
AAAATTCGTCAACTTCTTTTAAATGAAACAATGGAACCTCTAACTGAAGC
TTATTTGTTTGCCGCAGCTAGAACTGAACATATCAGTAAGCTAATTAAAC
CAGCAATTGAAAAAGAACAACTAGTTATTTCAGATAGATTTGTTTTCTCT
AGTTTTGCATACCAAGGATTAAGCAAAAAAATAGGCATTGATACAGTAAA
ACAGATTAATCATCATGCGTTAAGAAATATGATGCCAAACTTTACCTTTA
TTTTGGATTGCAATTTTAAAGAAGCATTACAAAGGATGCAAAAGCGTGGT
AATGATAATCTTCTTGATGAATTTATTAAAGGAAAGAATGATTTTGATAC
AGTTCGTTCTTATTATTTAAGCTTAGTTGATAAAAAAAACTGTTTCTTGA
TTAATGGTGATAATAAACAAGAACACCTAGAGAAATTTATTGAATTGTTA
ACAAGATGCTTACAACAACCCACGCATTAC


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