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Haemophilus ducreyi Search Results

Record: 1 of 1  
MiniMap IGR29 IGR24 IGR25 IGR23 IGR27 IGR21 IGR28 IGR26 IGR20 IGR22 genX, - HD0029 dcuB, - HD0025 HD0035 frdD, - HD0034 frdC, - HD0033 HD0038 frdB, - HD0032 dapF, - HD0026 lysA, - HD0036 frdA, - HD0030 hemN, - HD0037 genX, - HD0029 dcuB, - HD0025 HD0035 frdD, - HD0034 frdC, - HD0033 HD0038 frdB, - HD0032 dapF, - HD0026 lysA, - HD0036 frdA, - HD0030 hemN, - HD0037 genX, - HD0029 dcuB, - HD0025 HD0035 frdC, - HD0033 HD0038 frdB, - HD0032 dapF, - HD0026 lysA, - HD0036 frdA, - HD0030 hemN, - HD0037 frdD, - HD0034
* Calculated from Protein Sequence

Gene ID: HD0032

DNA Molecule Name:

Genbank ID:

Gene Name:

fumarate reductase iron-sulfur protein

Gene Start:

Gene Stop:

Gene Length:

Molecular Weight*:


Net Charge*:


Functional Class:
Energy metabolism; Anaerobic respiration  

Pathway: pathway table
Butanoate metabolism
Citrate cycle (TCA cycle)
Oxidative phosphorylation
Reductive carboxylate cycle (CO2 fixation)

Secondary Evidence:
Nucleotide sequence coding for the flavoprotein subunit the fumarate reductase of Escherichia coli.
Eur. J. Biochem. 122 (3), 479-484 (1982)
Medline: 82138876.

Blaut,M., Whittaker,K., Valdovinos,A., Ackrell,B.A., Gunsalus,R.P. and Cecchini,G.
Fumarate reductase mutants of Escherichia coli that lack covalently bound flavin.
J. Biol. Chem. 264 (23), 13599-13604 (1989)
Medline: 89340438.

Schroder,I., Gunsalus,R.P., Ackrell,B.A., Cochran,B. and Cecchini,G.
Identification of active site residues of Escherichia coli fumarate reductase by site-directed mutagenesis.
J. Biol. Chem. 266 (21), 13572-13579 (1991)
Medline: 91310624.

Iverson,T.M., Luna-Chavez,C., Cecchini,G. and Rees,D.C.
Structure of the Escherichia coli fumarate reductase respiratory complex.
Science 284 (5422), 1961-1966 (1999)
Medline: 99301923.

Cole,S.T., Grundstrom,T., Jaurin,B., Robinson,J.J. and Weiner,J.H. Location and nucleotide sequence of frdB, the gene coding for the iron-sulphur protein subunit of the fumarate reductase of Escherichia coli.
Eur. J. Biochem. 126 (1), 211-216 (1982)
Medline: 83027298.

Werth,M.T., Cecchini,G., Manodori,A., Ackrell,B.A., Schroder,I., Gunsalus,R.P. and Johnson,M.K.
Site-directed mutagenesis of conserved cysteine residues in Escherichia coli fumarate reductase: modification of the spectroscopic and electrochemical properties of the [2Fe-2S] cluster.
Proc. Natl. Acad. Sci. U.S.A. 87 (22), 8965-8969 (1990)
Medline: 91062400

From GenBank (gi:120492): In E. coli, two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth and the succinate dehydrogenase is used in aerobic growth. Catalytic activity of this protein in E. coli is: succinate + acceptor = fumarate + reduced acceptor, requiring cofactors (binds three different iron-sulfur clusters: a 2FE-2S, a 3FE-4S and a 4FE-4S). The iron-sulfur centers are similar to those of "plant-type" 2FE-2S and "bacterial-type" 4FE-4S ferredoxins. Fumerate dehydrogenase forms part of an enzyme complex containing four subunits: a flavoprotein (HD0030), an iron-sulfur protein (see HD0032), and two hydrophobic anchor proteins (see HD0033 and HD0034).

Blast Summary:  PSI-Blast Search
Numerous significant hits in gapped BLAST to fumarate reductase iron-sulfur proteins; e.g., residues 1-244 are 81% similar to FRDB_HAEIN [1169738], residues 1-243 are 73% similar to FRDB_ECOLI [120492], and residues 4-243 are 72% similar to FRDB_PROVU [120493].

COGS Summary:  COGS Search
BeTs to 12 clades of COG0479
COG name: Succinate dehydrogenase/fumarate reductase Fe-S protein
Functional Class:  C
The phylogenetic pattern of COG0479 is amt-yQ-CEbRhuj----inx
Number of proteins in this genome belonging to this COG is 1

Blocks Summary:  Blocks Search
***** IPB000564 (2Fe-2S Ferredoxin) with a combined E-value of 1.5e-08.
    IPB000564A    55-73
    IPB000564B    76-85

ProDom Summary:  Protein Domain Search
Residues 146-225 are 37% similar to a (IRON-SULFUR SUCCINATE DEHYDROGENASE) protein domain (PD375410) which is seen in Q9JRX8_CHLPN.

Residues 145-220 are 47% similar to a (IRON-SULFUR PROTEOME COMPLETE REDUCTASE) protein domain (PD016240) which is seen in O29575_ARCFU.

Residues 23-142 are 76% similar to a (IRON-SULFUR DEHYDROGENASE SUCCINATE) protein domain (PD001472) which is seen in Q9CP57_PASMU.

Residues 182-228 are 82% similar to a (IRON-SULFUR DEHYDROGENASE SUCCINATE) protein domain (PD000788) which is seen in Q9CP57_PASMU.

Paralogs:  Local Blast Search

HD0032 has no significant similarity (blastp p-value < 1e-3) to any other gene in this genome.

Pfam Summary:  Pfam Search
No significant hits to the Pfam 11.0 database

PDB Hit:
pdb|1FUM|B Chain B, Structure Of The E. Coli Fumarate Reductase... 385 3e-108
pdb|1QLA|B Chain B, Respiratory Complex Ii-Like Fumarate Reduct... 127 1e-030

Gene Protein Sequence:

Gene Nucleotide Sequence:  Sequence Viewer

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