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Chlamydia pneumoniae Search Results

Record: 1 of 1  
MiniMap IGR684 IGR685 IGR683 IGR686 fliF, - CPn0860 ompB, - CPn0854 yfhO,nifS, - CPn0862 fliI, - CPn0858 CPn0856 CPn0853 fliF, - CPn0860 ompB, - CPn0854 yfhO,nifS, - CPn0862 fliI, - CPn0858 CPn0856 CPn0853 fliF, - CPn0860 ompB, - CPn0854 yfhO,nifS, - CPn0862 fliI, - CPn0858 CPn0856 CPn0857 CPn0859 pgmA, - CPn0863 nifU, - CPn0861 gpdA  gpsA, - CPn0855 CPn0857 CPn0859 pgmA, - CPn0863 nifU, - CPn0861 gpdA  gpsA, - CPn0855 CPn0853
* Calculated from Protein Sequence

Gene ID: CPn0858

DNA Molecule Name:
1  

Genbank ID:


Gene Name:
fliI  

Definition:
probable flagellar-type ATP synthase (beta subunit)

Gene Start:
977055

Gene Stop:
975754

Gene Length:
1302

Molecular Weight*:
47469

pI*:
8.00

Net Charge*:
5.64

EC:
 

Functional Class:
cellular processes; secretion  

Pathway: pathway table

Comment:
From Prosite PDOC00137:

TP synthase (proton-translocating ATPase) (EC 3.6.1.34) is a component of the cytoplasmic membrane of eubacteria, the inner membrane of mitochondria, and the thylakoid membrane of chloroplasts. The ATPase complex is composed of an oligomeric transmembrane sector, called CF(0), and a catalytic core, called coupling factor CF(1). The former acts as a proton channel; the latter is composed of five subunits, alpha, beta, gamma, delta and epsilon. The sequences of subunits alpha and beta are related and both contain a nucleotide-binding site for ATP and ADP. The beta chain has catalytic activity, while the alpha chain is a regulatory subunit.

Vacuolar ATPases (V-ATPases) are responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Like F-ATPases, they are oligomeric complexes of a transmembrane and a catalytic sector. The sequence of the largest subunit of the catalytic sector (70 Kd) is related to that of F-ATPase beta subunit, while a 60 Kd subunit, from the same sector, is related to the F-ATPases alpha subunit.

Archaebacterial membrane-associated ATPases are composed of three subunits. The alpha chain is related to F-ATPases beta chain and the beta chain is related to F-ATPases alpha chain.

A protein highly similar to F-ATPase beta subunits is found in some bacterial apparatus involved in a specialized protein export pathway that proceeds without signal peptide cleavage. This protein is known as fliI in Bacillus and Salmonella, Spa47 (mxiB) in Shigella flexneri, HrpB6 in Xanthomonas campestris and yscN in Yersinia virulence plasmids.

In order to detect these ATPase subunits, we took a segment of ten amino-acid residues, containing two conserved serines, as a signature pattern. The first serine seems to be important for catalysis - in the ATPase alpha chain at least - as its mutagenesis causes catalytic impairment.

Blast Summary:  PSI-Blast Search
Numerous significant hits in gapped BLAST to flagellar-type ATP synthase sequences; e.g. residues 1-427 are 68% similar to CT717; residues 4-430 are 36% similar to TP0402; residues 23-430 are 36% similar to CT669; residues 17-430 are 34% similar to FLII_BACSU; and residues 19-427 are 34% similar to (AF069392) polar flagellum-specific ATP synthase [Vibrio parahaemolyticus].

COGS Summary:  COGS Search
BeTs to 8 clades of COG1157
COG name: Flagellar ATPase-like component
Functional Class:  C
The phylogenetic pattern of COG1157 is -----qv-eb--uj--olIN-
Number of proteins in this genome belonging to this COG is 2

Blocks Summary:  Blocks Search
Residues 86-111, 143-180, 233-244, and 252-296 represent blocks BL00152A-D (ATP synthase alpha and beta subunits proteins) with a combined E-value of 8.2e-38.

ProDom Summary:  Protein Domain Search
Residues 96-198 are 50% similar to a defined domain of O52140_ECOLI, defined as: ATP TRANSPORT HYDROLASE ION HYDROGEN SYNTHASE ATP-BINDING SYNTHESIS CHAIN SUBUNIT.
Residues 300-430 are 35% similar to a defined domain of FLII_SALTY, defined as: ATP TRANSPORT HYDROLASE ION HYDROGEN SYNTHASE SYNTHESIS ATP-BINDING CHAIN SUBUNIT.

Paralogs:  Local Blast Search
CPn0707, CPn0089, CPn0088, and CPn0610 are possible paralogs.
Residues 21-430 are 36% similar to CPn0707.

Pfam Summary:  Pfam Search
Residues 23 to 87 (E-value = 1.9e-07) place CPn0858 in the ATP-synt_ab_N family which is described as ATP synthase alpha/beta family, beta-barrel domain (PF02874)
Residues 183 to 357 (E-value = 1.5e-48) place CPn0858 in the ATP-synt_ab family which is described as ATP synthase alpha/beta family, nucleotide-binding domain (PF00006)

Structural Feature(s):
Feature Type  Start  Stop
non-globular  
52  
132
non-globular  
304  
433

PDB Hit:


Gene Protein Sequence:
MNHLNKEKLHIHNWQPYRACGLLSKVSGNLIEVDGLSACLGELCKISSTK
DPNLLAEVIGFHNHTTLLMSLSPLHSVALGTEVLPLRRPPSLHLSDHLLG
RVLDAFGNPIDKKEDLPKTHRKPLLSLPPSPMMRQPIDQIFPTGIKAIDA
FLTLGKGQRIGVFSEPGSGKSSLLSAIALGSKSTINVIALIGERGREVRE
YIEKHSNALKQQRTIIIAAPAHETAPTKVIAGRAAMTIAEYFREQGHEVL
FIMDSLSRWIAALQEVALARGETLSAHQYAASVFHHVSEFTERAGNNDKG
SITALYAILYYPKHPDIFTDYLKSLLDGHFFLTSQGKALASPPIDILSSL
SRSAQALALPHHYAAAERLRSLLKVYNEALDIIHLGAYTPGQDEELDKAV
KLLPSIKAFLAQPLSSYCYLDNTLKQLEALADS$

Gene Nucleotide Sequence:  Sequence Viewer
ATGAATCATCTAAATAAAGAAAAACTTCATATTCATAACTGGCAGCCCTA
CCGTGCCTGTGGTCTATTATCTAAGGTCTCTGGAAATCTTATTGAGGTCG
ATGGGCTCTCTGCATGCCTGGGGGAGCTCTGCAAAATTTCCTCAACCAAA
GATCCGAATCTTCTTGCTGAAGTGATTGGCTTTCACAATCACACGACACT
TCTCATGTCCCTATCTCCGTTACACTCTGTAGCTCTTGGAACAGAAGTCC
TTCCTTTACGCCGCCCCCCCTCTCTACATCTCTCAGATCATCTTTTAGGG
AGAGTCTTAGATGCTTTCGGCAACCCTATTGACAAGAAAGAGGACCTTCC
TAAAACACATCGAAAACCTCTTCTGTCTCTACCGCCATCCCCTATGATGA
GACAACCTATCGATCAGATCTTCCCTACAGGGATTAAAGCAATCGATGCA
TTTCTTACCTTAGGGAAAGGACAGCGTATTGGAGTTTTCTCTGAGCCAGG
AAGTGGGAAGTCTTCACTGCTCTCAGCAATCGCTTTAGGATCAAAATCTA
CAATCAATGTGATTGCTTTAATTGGAGAGCGGGGACGCGAAGTCCGAGAA
TACATAGAGAAGCATAGCAATGCCCTGAAACAGCAGCGTACGATCATCAT
TGCAGCACCTGCCCATGAGACTGCACCAACAAAAGTAATCGCAGGGCGAG
CGGCTATGACAATAGCAGAATATTTTCGAGAGCAAGGCCATGAAGTCTTA
TTTATTATGGACTCTTTATCCCGATGGATCGCTGCACTCCAAGAAGTCGC
TTTAGCGCGAGGAGAAACACTCTCTGCGCACCAATATGCCGCCTCTGTCT
TTCACCATGTATCTGAATTTACAGAACGTGCCGGAAACAATGACAAGGGG
TCCATAACAGCTCTGTATGCGATTTTATATTACCCGAAGCATCCTGATAT
TTTTACAGACTATTTAAAATCGCTGTTGGATGGACACTTTTTCCTAACAA
GCCAAGGTAAAGCCTTAGCCTCCCCCCCAATCGATATTTTATCAAGTCTT
TCTCGGTCAGCACAAGCACTAGCACTTCCCCATCATTATGCAGCTGCAGA
AAGACTTCGTTCACTACTTAAGGTATATAATGAAGCTTTAGATATCATTC
ATCTAGGAGCTTACACTCCCGGTCAAGATGAAGAGTTAGATAAAGCAGTG
AAGCTCCTTCCCAGCATCAAAGCCTTCCTAGCGCAACCCTTGTCTAGTTA
CTGTTACTTAGACAATACATTAAAACAGCTCGAGGCATTAGCAGACTCAT
GA


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